Site-Resolved Determination of Peptide Torsion Angle φ from the Relative Orientations of Backbone N−H and C−H Bonds by Solid-State NMR

Determination of the Peptide Torsion Ang

Determination of the Peptide Torsion Angle φ by15N Chemical Shift and13Cα-1HαDipolar Tensor Correlation in Solid-State MAS NMR

✍ M. Hong; J.D. Gross; W. Hu; R.G. Griffin 📂 Article 📅 1998 🏛 Elsevier Science 🌐 English ⚖ 214 KB

We demonstrate a dipolar-chemical shift correlation technique for sign-sensitive determination of the torsion angle in solid peptides and proteins under magic-angle spinning. The indirect dimension of the experiment is obtained by separate but synchronous evolution of the magnetization under the 15