Hydration of protein cavities influences protein stability, dynamics, and function. Protein active sites usually contain water molecules that, upon ligand binding, are either displaced into bulk solvent or retained to mediate protein-ligand interactions. The contribution of water molecules to ligand
β¦ LIBER β¦
Site-directed mutants of cytochrome P450cam: Role of THR252 in the catalysis
β Scribed by Y. Ishimura; H. Shimada; Y. Kimata; T. Hirose
- Publisher
- Elsevier Science
- Year
- 1995
- Tongue
- English
- Weight
- 78 KB
- Volume
- 59
- Category
- Article
- ISSN
- 0162-0134
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## Abstract __The effects of siteβspecific mutation of the axial cysteine (C357M) to a methionine residue in cytochrome P450cam on the enzymeβs coordination geometry and redox potential have been investigated. The absorption spectra of the haem centre in the C357M mutant of the enzyme showed close
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