Site-directed mutagenesis study of the antibody 2D7 which catalyzes a reaction for insertion of Cu2+ into mesoporphyrin

Exploration of the S3.0.CO;2-T/asset/equ

Exploration of the S3.0.CO;2-T/asset/equation/tex2gif-stack-1.gif?v=1&t=h5logaf2&s=c21bccb7a61f29aec9763209954754d152394698" class="inlineGraphic"> subsite of neprilysin: A joined molecular modeling and site-directed mutagenesis study

✍ Cynthia Marie-Claire; Gilles Tiraboschi; Emmanuel Ruffet; Nicolas Inguimbert; Ma 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 300 KB 👁 1 views

Based on the recently described three-dimensional model of the 507-749 region of neprilysin, which contains the catalytic site of the enzyme, experiments were performed to improve the proposed topology of its large and hydrophobic S 1 subsite. Docking studies, site-directed mutagenesis, and biochemi