Altered product pattern of a squalene-ho
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Thorsten Merkofer; Catherine Pale-Grosdemange; Karl Ulrich Wendt; Michel Rohmer;
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Article
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1999
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Elsevier Science
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French
⚖ 177 KB
Amino acid residues lining the catalytic cavity of squalene-hopene cyclase of Alicyclobacillus acidocaldarius have been mutated. Alterations of His451 to Ala and Trp489 to Ala resulted in reduced enzymatic activity, while the product patterns were identical to that of the wild-type. Mutation of Phe6