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Site-Directed Mutagenesis Clarifies the Substrate Position within the Three-Dimensional Model of the Active Site of Herpes Simplex Virus Type-1 Thymidine Kinase

✍ Scribed by Martina Michael; Jürgen Fetzer; Gerd Folkers

Book ID: 115130603
Publisher: John Wiley and Sons
Year: 2008
Tongue: English
Weight: 731 KB
Volume: 226
Category: Article
ISSN: 1432-1327
DOI: 10.1111/j.1432-1033.1994.0t219.x

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## Abstract To analyze the site of integration of the herpes simplex virus type 1 (HSV‐1) thymidine kinase (TK) gene in biochemically transformed human cells, TK‐HeLa‐(BU25) cells were transformed to the TK^+^ phenotype by a cloned, 2 kbp Pvull fragment of HSV‐1 DNA. The transformed cells [HeLa(BU2