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Site-Directed Mutagenesis and Chemical Modification of the Six Native Cysteine Residues of the Rat Mitochondrial Carnitine Carrier: Implications for the Role of Cysteine-136 †

✍ Scribed by Indiveri, Cesare; Giangregorio, Nicola; Iacobazzi, Vito; Palmieri, Ferdinando


Book ID
126075361
Publisher
American Chemical Society
Year
2002
Tongue
English
Weight
85 KB
Volume
41
Category
Article
ISSN
0006-2960

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## Abstract A cDNA of rat liver thioltransferase was cloned and then expressed using pMAL‐c expression vector in __Escherichia coli__. Recombinant rat liver thioltransferase was expressed as a fusion protein with maltose‐binding protein and then purified by amylose resin column chromatography to be