Site-directed mutagenesis of rat thioltr
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Tomoyuki Terada; Kei-ichi Okamoto; Jun-ichi Nishikawa; Takeshi Miura; Toru Nishi
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Article
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2010
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John Wiley and Sons
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English
⚖ 116 KB
## Abstract A cDNA of rat liver thioltransferase was cloned and then expressed using pMAL‐c expression vector in __Escherichia coli__. Recombinant rat liver thioltransferase was expressed as a fusion protein with maltose‐binding protein and then purified by amylose resin column chromatography to be