We present two examples of exponential nucleic acid amplification with the polymerase chain reaction (PCR) in the presence of only one amplification primer. Cloning and sequencing of the PCR products generated by amplification of human genomic DNA revealed that the amplified sequence contained only
β¦ LIBER β¦
Single-step single-molecule PCR of DNA with a homo-priming sequence using a single primer and hot-startable DNA polymerase
β Scribed by Hideo Nakano; Koei Kobayashi; Shoji Ohuchi; Satoshi Sekiguchi; Tsuneo Yamane
- Book ID
- 114398932
- Publisher
- Elsevier Science
- Year
- 2000
- Tongue
- English
- Weight
- 398 KB
- Volume
- 90
- Category
- Article
- ISSN
- 1389-1723
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An optimized reaction condition for amplification of influenza A virus RNA, by thermus thermophilus (Tth) DNA polymerase-based PCR, incorporating uracil N glycosylase (UNG) and dUTP in the reaction has been determined. dUTP could not be substituted for all dTTP sites when UNG was present in the reac