✦ LIBER ✦
Single-step separation of major and rare ribonucleosides and deoxyribonucleosides by high-performance liquid cation-exchange chromatography for the determination of the purity of nucleic acid preparations
✍ Scribed by Hans-Joachim Breter; Gerhard Seibert; Rudolf K. Zahn
- Publisher
- Elsevier Science
- Year
- 1977
- Tongue
- English
- Weight
- 380 KB
- Volume
- 140
- Category
- Article
- ISSN
- 1873-3778
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✦ Synopsis
A method is described for the separation of 13 major and minor ribo-and deoxyribonucleosides in a single chromatographic run using high-performance liquid chromatography on strongly acidic cation-exchange columns. The method proved useful for the routine determination of small amounts of ribonucleic acid impurities in deoxyribonucleic acid preparations and vice versa. About 3 o/o or even less of nucleic acid contamination in a given sample can be easily detected and quantitatively determined under the conditions used.