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Single-step selection of mammalian cell mutants deficient in CTP synthetase

โœ Scribed by I-Chian Li; Chao-Liang Wu; Ernest H. Y. Chu


Book ID
112557712
Publisher
Springer
Year
1989
Tongue
English
Weight
413 KB
Volume
15
Category
Article
ISSN
1572-9931

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Stable mutants of Chinese hamster V79 cells deficient in thymidylate synthetase (TS; E.C. 2.1.1.45) have been selected from cultures grown in medium supplemented with folinic acid, aminopterin, and thymidine (FAT). After chemical mutagenesis, the frequency of colonies resistant to the "FAT" medium i