Single session to infrared low level diode laser on TNF-α and IL-6 cytokines release by mononuclear spleen cells in mice: A pilot study
✍ Scribed by Thiago Y. Fukuda; Maury M. Tanji; Julio F. Jesus; Maria N. Sato; Alberto J.S. Duarte; Hélio Plapler
- Publisher
- John Wiley and Sons
- Year
- 2010
- Tongue
- English
- Weight
- 84 KB
- Volume
- 42
- Category
- Article
- ISSN
- 0196-8092
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✦ Synopsis
Abstract
Background and Objective
The results of low‐level infrared laser (LLL) systemic action on inflammatory modulation process, specifically diminishing pro‐inflammatory and producing anti‐inflammatory cytokines are extremely controversial in the literature. More studies are necessary to clarify the biomodulation process. The main objective was to investigate the effect of a single session of an AsGaAl laser on spleen cells interleukin‐6 (IL‐6) and tumor necrosis factor ‐ alpha (TNF‐α) release, in vivo, in mice.
Study Design/Materials and Methods
In a pilot study, 18 isogenic mice were distributed in three groups: control (no surgical procedure, n = 6), sham (surgical procedure with three standard cutaneous incisions, followed by abdominal muscle incision followed by suture, n = 6) and LLL (same procedure followed by a single LLL exposure 12 hours after the procedure, n = 6). The animals in the LLL group received a single infrared continuous laser session (780 nm wavelength, power of 20 mW, energy density of 10 J/cm^2^) on three points (20 seconds per point), and final energy of 0.4 J. All animals of the sham and LLL groups were sacrificed 36 hours after surgical procedure; the spleen mononuclear cells were isolated and cultivated for 48 hours. The IL‐6 and TNF‐α were measured by the ELISA method.
Results
IL‐6 and TNF‐α concentrations released by the mononuclear cells showed significant differences between the control and sham group (P < 0.07). However, there were no differences between the control and LLL group and between the sham and LLL groups (P > 0.07).
Conclusion
The single session of infrared LLL showed a tendency of decreasing the IL‐6 and TNF‐α release by mononuclear spleen cells in mice after application, although there was not a significant difference between the sham and LLL group. Conclusions regarding effectiveness of a single session procedure cannot be made due to the low statistical power of this pilot study. Lasers Surg. Med. 42:584–588, 2010. © 2010 Wiley–Liss, Inc.