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Single nucleotide sequence analysis: a cost- and time-effective protocol for the analysis of microsatellite- and indel-rich chloroplast DNA regions

✍ Scribed by DANIELA GUICKING; TIM KRÖGER-KILIAN; KURT WEISING; FRANK R. BLATTNER


Book ID
111188919
Publisher
Wiley (Blackwell Publishing)
Year
2008
Tongue
English
Weight
113 KB
Volume
8
Category
Article
ISSN
1755-098X

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Tobacco chloroplast DNA contains a large inverted repeat sequence of 26 kilobase pairs (kbp). The inverted repeat is separated by 20 kbp small single-copy and 90 kbp large single-copy regions. We have cloned four DNA fragments containing each junction between the inverted repeat and the single-copy