Single nucleotide polymorphisms (SNPs) within Bov-A2 SINE in the second intron of bovine and buffalo k-casein (CSN3) gene
✍ Scribed by G Damiani; S Florio; E Budelli; P Bolla; A Caroli
- Publisher
- John Wiley and Sons
- Year
- 2000
- Tongue
- English
- Weight
- 144 KB
- Volume
- 31
- Category
- Article
- ISSN
- 0268-9146
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✦ Synopsis
The genetic polymorphism of an entire Bov‐A2 element located in the second intron of the buffalo and bovine k‐casein (CSN3) gene was investigated by amplification and sequencing of PCR products. Single nucleotide polymorphisms were detected. A PCR‐RFLP method was developed to detect an A or G mutation at position 605 of bovine Bov‐A2 element which creates a __Bfa__I polymorphic site. The frequencies of the B allele, with the __Bfa__I site, were for 0·275, 0·775, 0·750, 0·975, respectively, for Italian Holstein Friesian, Grey Alpine, Friuli Red Pied and Reggio bovine breeds. The mutation rate (substitutions and deletions/insertions per nucleotide site per year) was 2·5 × 10−9 for Bov‐A2 sequences in the second intron of CSN3. The comparison with other Bov‐A2 elements suggests that this retroelement might be an important source of single nucleotide polymorphism for analysis of Bovidae genomes.