A method for typing single nucleotide polymorphisms (SNPs) by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) is described, in which a mass-tagged dideoxynucleoside triphosphate is employed in a primer extension reaction in place of an unmodified dideoxynuc
Single-nucleotide polymorphisms: analysis by mass spectrometry
β Scribed by Sauer, Sascha; Reinhardt, Richard; Lehrach, Hans; Gut, Ivo G
- Book ID
- 109945884
- Publisher
- Nature Publishing Group
- Year
- 2006
- Tongue
- English
- Weight
- 335 KB
- Volume
- 1
- Category
- Article
- ISSN
- 1750-2799
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## Abstract | βI. | Introduction | 389 | | | A. A Brief History of DNA Analysis by Mass Spectrometry | 389 | | | B. What Exactly Are SNPs | 390 | | | C. Why Use MALDI for DNA Analysis? | 391 | | II. | Methods for AlleleβDiscrimination | 391 | | | A. Hybridization | 391 | | | B. Oligonucleotide
There is a growing demand for high-throughput methods for analysis of single-nucleotide polymorphic (SNP) positions. Here, we have evaluated a novel sequencing approach, pyrosequencing, for such purposes. Pyrosequencing is a sequencing-by-synthesis method in which a cascade of enzymatic reactions yi