Single nucleotide polymorphism wars
โ Scribed by Chauvet, Jean-Marie
- Book ID
- 109901891
- Publisher
- Nature Publishing Group
- Year
- 1998
- Tongue
- English
- Weight
- 216 KB
- Volume
- 16
- Category
- Article
- ISSN
- 1087-0156
No coin nor oath required. For personal study only.
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Large-scale sequencing of human cDNA and genomic DNA libraries has produced a large collection of sequence data in public databases. To date, >900,000 human expressed sequence tag (EST) sequences and >80,000,000 bases of genomic DNA sequence have been deposited in Genbank. This ever-expanding data s
There is a growing demand for high-throughput methods for analysis of single-nucleotide polymorphic (SNP) positions. Here, we have evaluated a novel sequencing approach, pyrosequencing, for such purposes. Pyrosequencing is a sequencing-by-synthesis method in which a cascade of enzymatic reactions yi