Single-Molecule Quantum-Dot Fluorescence Resonance Energy Transfer

✍ Scribed by Sungchul Hohng; Taekjip Ha

• Publisher: John Wiley and Sons
• Year: 2005
• Tongue: English
• Weight: 163 KB
• Volume: 6
• Category: Article
• ISSN: 1439-4235
• DOI: 10.1002/cphc.200400557

✦ Synopsis

Abstract

Colloidal semiconductor quantum dots are promising for single‐molecule biological imaging due to their outstanding brightness and photostability. As a proof of concept for single‐molecule fluorescence resonance energy transfer (FRET) applications, we measured FRET between a single quantum dot and a single organic fluorophore Cy5. DNA Holliday junction dynamics measured with the quantum dot/Cy5 pair are identical to those obtained with the conventional Cy3/Cy5 pair, that is, conformational changes of individual molecules can be observed by using the quantum dot as the donor.