Single-Molecule Microscopy of the Green Fluorescent Protein Using Simultaneous Two-Color Excitation
✍ Scribed by Gregor Jung; Jens Wiehler; Boris Steipe; Christoph Bräuchle; Andreas Zumbusch
- Publisher
- John Wiley and Sons
- Year
- 2001
- Tongue
- English
- Weight
- 132 KB
- Volume
- 2
- Category
- Article
- ISSN
- 1439-4235
No coin nor oath required. For personal study only.
📜 SIMILAR VOLUMES
Background: Development of spectrally distinct green fluorescent protein (GFP) variants has allowed for simultaneous flow cytometric detection of two different colored mutants expressed in a single cell. However, the dual-laser methods employed in such experiments are not widely applicable since the
The ability to analyze independently the expression of multiple reporter gene constructs within single cells is a potentially powerful application of flow cytometry. In this paper, we explore the simultaneous detection of two variants of the reporter molecule, green fluorescent protein (GFP) that bo