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Single-molecule measurements of trapped and migrating circular DNA during electrophoresis in agarose gels

✍ Scribed by Kenneth D. Cole; Adolfas Gaigalas; Björn Åkerman


Publisher
John Wiley and Sons
Year
2006
Tongue
English
Weight
483 KB
Volume
27
Category
Article
ISSN
0173-0835

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The use of single cell gel electrophoresis (SCGE) has recently been applied to plant systems. We optimized the experimental conditions for SCGE analysis using nuclei isolated from different tissues of intact plants. Concentration-response curves of genomic DNA migration were analyzed in intact plant