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Single-molecule fluorescence imaging of membrane-bound proteins for studies of cell signal transduction

✍ Scribed by Yong Yang; Tie Xia; Wei Zhang; XiaoHong Fang

Book ID
107372525
Publisher
Springer
Year
2011
Tongue
English
Weight
612 KB
Volume
56
Category
Article
ISSN
1001-6538

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## Abstract A new algorithm is presented for the detection of single, fluorescence‐labeled proteins in the analysis of images from living cells. It is especially suited for images with just a few (<1 per 10 ΞΌm^2^) fluorescence peaks from individual proteins with high background and noise (signal to