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Single molecule detection of RNA reporter probes by amplification with Qβ replicase

✍ Scribed by Lawrence J. Burg; Ann Marie Juffras; Ye Wu; Cecile L. Blomquist; Yan Du


Book ID
115638121
Publisher
Elsevier Science
Year
1996
Tongue
English
Weight
224 KB
Volume
10
Category
Article
ISSN
0890-8508

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Real-Time Fluorescence Detection of RNA
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Amplification of RNA probes by \(\mathbf{Q \beta}\) replicase can be used to detect a wide range of analytes with a potential sensitivity of a single molecule. A system has been developed in which \(Q \beta\) amplification of midivariant(MDV)-based RNA is measured in real time by fluorescence. This