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Single Molecule Detection of DNA Looping by NgoMIV Restriction Endonuclease

✍ Scribed by Katiliene, Zivile; Katilius, Evaldas; Woodbury, Neal W.


Book ID
118515866
Publisher
Biophysical Society
Year
2003
Tongue
English
Weight
292 KB
Volume
84
Category
Article
ISSN
0006-3495

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A model for kinetics of circular substrate cleavage by restriction endonuclease was formulated. The aim of the analysis of the model was to extract kinetic constants for all target sites from time-dependence of fragment concentration in reaction products. That was proved to be possible for molecules