The detection of single molecules opens up new horizons in analytical chemistry, biology and medicine. This discipline, which belongs to the expanding field of nanoscience, has been rapidly emerging over the last ten years.
This handbook provides a thorough overview of the field. It begins with basics of single molecule detection in solution, describes methods and devices (fluorescense correlation spectroscopy, surface enhanced Raman scattering, sensors, especially dyes, screening techniques, especially confocal laser scanning microscopy). In the second part, various applications in life sciences and medicine provide the latest research results.
This modern handbook is a highly accessible reference for a broad community from advanced researchers, specialists and company professionals in physics, spectroscopy, biotechnology, analytical chemistry, and medicine. Written by leading authorities in the field, it is timely and fills a gap - up to now there exists no handbook concerning this theme.
Content:
Chapter 1 Single Molecule Detection in Liquids and on Surfaces under Ambient Conditions: Introduction and Historical Overview (pages 1โ19): Jorg Enderlein, Richard A. Keller and Christoph Zander
Chapter 2 Theoretical Foundations of Single Molecule Detection in Solution (pages 21โ67): Jorg Enderlein and Christoph Zander
Chapter 3 Conceptual Basis of Fluorescence Correlation Spectroscopy and Related Techniques as Tools in Bioscience (pages 69โ120): Jerker Widengren and Ulo Mets
Chapter 4 Surface?Enhanced Raman Scattering (SERS) โ A Tool for Single Molecule Detection in Solution (pages 121โ144): Katrin Kneipp, Harald Kneipp, Irving Itzkan, Ramachandra R. Dasari and Michael S. Feld
Chapter 5 Single Molecule Detection on Surfaces with the Confocal Laser Scanning Microscope (pages 145โ183): Martin Bohmer and Jorg Enderlein
Chapter 6 Spectroscopy of Individual Photosynthetic Pigment?Protein Complexes (pages 185โ229): J. Wrachtrup, T.J. Aartsma, J. Kohler, M. Ketelaars, A. M. van Oijen, M. Matsushita, J. Schmidt, C. Tietz and F. Jelezko
Chapter 7 Single Dye Tracing for Ultrasensitive Microscopy on Living Cells (pages 231โ245): Gerhard J. Schutz and Hansgeorg Schindler
Chapter 8 Single Molecule Identification in Solution: Principles and Applications (pages 247โ272): M. Sauer and C. Zander
Chapter 9 Studying Molecular Motors on the Single Molecule Level (pages 273โ292): Y. Ishii, A. H. Iwane, H. Yokota, Y. Inoue, T. Wazawa, M. Nishiyama, H. Tanaka, K. Kitamura and T. Yanagida
Chapter 10 The Chemistry of a Single Enzyme Molecule (pages 293โ301): Robert Polakowski, Michael Eggertson, Douglas B. Craig and Norman J. Dovichi
Chapter 11 Single Molecule Detection of Specific Nucleic Acid Sequences (pages 303โ321): Alonso Castro
Chapter 12 Single Molecule Detection in the Near?Infrared (pages 323โ362): Steven A. Soper, Musundi Wabuyele, Clyde V. Owens and Robert P. Hammer