Single molecule analysis : methods and protocols

✍ Scribed by Peterman, Erwin J. G

Publisher: Humana Press ; Springer
Year: 2018
Tongue: English
Leaves: 369
Series: Methods in molecular biology 1665
Edition: Second edition
Category: Library

No coin nor oath required. For personal study only.

✦ Synopsis

The aim of this volume is to provide a broad overview of single-molecule approaches applied to biomolecules. Chapters in Single Molecule Techniques: Methods and Protocols, Second Edition detail the most widely used single-molecule techniques, such as DNA, DNA-binding proteins, motor proteins, and are becoming commonplace in molecular biophysics, biochemistry, and molecular and cell biology. Written in the highly successful Methods in Molecular Biology series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Single Molecule Analysis: Methods and Protocols, Second Edition aims to be useful to many researchers, inspire them and help them to go single molecule.

✦ Table of Contents

Front Matter ....Pages i-xi
Front Matter ....Pages 1-1
Introduction to Optical Tweezers: Background, System Designs, and Commercial Solutions (Joost van Mameren, Gijs J. L. Wuite, Iddo Heller)....Pages 3-23
RNA Unzipping and Force Measurements with a Dual Optical Trap (Laurent Geffroy, Pierre Mangeol, Thierry Bizebard, Ulrich Bockelmann)....Pages 25-41
Protein Tethering for Folding Studies (Fatemeh Moayed, Roeland J. van Wijk, David P. Minde, Sander J. Tans)....Pages 43-51
Combining Structure–Function and Single-Molecule Studies on Cytoplasmic Dynein (Lu Rao, Maren Hülsemann, Arne Gennerich)....Pages 53-89
Front Matter ....Pages 91-91
A Brief Introduction to Single-Molecule Fluorescence Methods (Siet M. J. L. van den Wildenberg, Bram Prevo, Erwin J. G. Peterman)....Pages 93-113
Fluorescent Labeling of Proteins (Mauro Modesti)....Pages 115-134
Single-Molecule Imaging of Escherichia coli Transmembrane Proteins (Aravindan Varadarajan, Felix Oswald, Yves J. M. Bollen)....Pages 135-144
Single-Molecule Fluorescence Microscopy in Living Caenorhabditis elegans (Jaap van Krugten, Erwin J. G. Peterman)....Pages 145-154
Purification and Application of a Small Actin Probe for Single-Molecule Localization Microscopy (Roderick P. Tas, Trusanne G. A. A. Bos, Lukas C. Kapitein)....Pages 155-171
Fluorescence Microscopy of Nanochannel-Confined DNA (Fredrik Westerlund, Fredrik Persson, Joachim Fritzsche, Jason P. Beech, Jonas O. Tegenfeldt)....Pages 173-198
Use of Single Molecule Fluorescence Polarization Microscopy to Study Protein Conformation and Dynamics of Kinesin–Microtubule Complexes (Matthieu P. M. H. Benoit, Hernando Sosa)....Pages 199-216
Single Molecule FRET Analysis of DNA Binding Proteins (Kathy R. Chaurasiya, Remus T. Dame)....Pages 217-239
Front Matter ....Pages 241-241
Atomic Force Microscopy: An Introduction (Melissa C. Piontek, Wouter H. Roos)....Pages 243-258
Imaging of DNA and Protein by SFM and Combined SFM-TIRF Microscopy (Małgorzata Grosbart, Dejan Ristić, Humberto Sánchez, Claire Wyman)....Pages 259-280
Atomic Force Microscopy of Protein Shells: Virus Capsids and Beyond (Natalia Martín-González, Alvaro Ortega-Esteban, F. Moreno-Madrid, Aida Llauró, Mercedes Hernando-Pérez, Pedro J. de Pablo)....Pages 281-296
Combined Magnetic Tweezers and Micro-mirror Total Internal Reflection Fluorescence Microscope for Single-Molecule Manipulation and Visualization (Yeonee Seol, Keir C. Neuman)....Pages 297-316
Tethered Particle Motion: An Easy Technique for Probing DNA Topology and Interactions with Transcription Factors (Daniel T. Kovari, Yan Yan, Laura Finzi, David Dunlap)....Pages 317-340
Single-Molecule Measurements Using Acoustic Force Spectroscopy (AFS) (Douwe Kamsma, Gijs J. L. Wuite)....Pages 341-351
Repurposing a Benchtop Centrifuge for High-Throughput Single-Molecule Force Spectroscopy (Darren Yang, Wesley P. Wong)....Pages 353-366
Back Matter ....Pages 367-369

✦ Subjects

DNA -- Analysis -- Laboratory manuals;Single Molecule Imaging;Laboratory Manuals

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