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Single-cell gel (comet) assay detects primary DNA damage in peripheral blood lymphocytes of smokers

✍ Scribed by Karahan, Elif Gulsah; Tomatir, Ayse Gaye; Acikbas, Ibrahim; Er, Buket; Evyapan, Fatma; Akdag, Beyza; Arslan, Pervin Elvan


Book ID
126548643
Publisher
Elsevier Science
Year
2014
Tongue
English
Weight
83 KB
Volume
185
Category
Article
ISSN
0168-1656

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The single cell gel electrophoresis (SCGE) assay, or comet assay, is a highly sensitive method to measure DNA damage. The principle of the assay is based on the conversion of single-strand breaks and alkali-labile sites to double-strand breaks via alkaline lysis. The cells are subjected to electroph