Single cell assay of exocytosis from adrenal chromaffin cells using “perforated patch recording”

✍ Scribed by Kevin D. Gillis; Raymund Y. K. Pun; Stanley Misler

• Publisher: Springer
• Year: 1991
• Tongue: English
• Weight: 302 KB
• Volume: 418
• Category: Article
• ISSN: 0031-6768
• DOI: 10.1007/bf00370579

✦ Synopsis

We have combined "perforated patch recording" with phase detection to examine depolarization-induced changes in membrane capacitance (ACre) in bovine adrenal ehromaffin cells. With this technique, voltage dependent Ca 2+ currents and resultant ACre'S often show little rundown over 1-2 hours even when the free Ca 2+ concentration of the pipette is in the millimolar range. By limiting washout of cytosolic components and by maintaining more intact cytosolic Ca z § buffering, this approach should facilitate the study of stimulus-exocytosis coupling evoked by physiological stimuli which involve cell metabolism and/or membrane receptor triggered second messenger cascades.