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Single Base Pair Mismatch Detection Using Cationic Conjugated Polymers through Fluorescence Resonance Energy Transfer

✍ Scribed by Ning Tian; Yanli Tang; Qing-Hua Xu; Shu Wang

Publisher
John Wiley and Sons
Year
2007
Tongue
English
Weight
204 KB
Volume
28
Category
Article
ISSN
1022-1336

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✦ Synopsis

Abstract

A simple method using water‐soluble conjugated polymers and a DNA intercalator has been proposed for single base pair mismatch detection with enhanced detection efficiency. Fluorescence resonance energy transfer (FRET) was used as an indicator for unwinding of dsDNA due to base pair mismatch at elevated temperatures. The optical amplification effect of the CPs helps to achieve enhanced detection efficiency.

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