Single- and double-strand breaks in 5-bromouracil-substituted DNA of B. subtilis and phage PBSH after irradiation with long-wave length UV and their correlation to intramolecular energy transfer
✍ Scribed by Friedrich Mönkehaus; Wolfgang Köhnlein
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 1973
- Tongue
- English
- Weight
- 641 KB
- Volume
- 12
- Category
- Article
- ISSN
- 0006-3525
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✦ Synopsis
Abstract
5‐Bromouracil‐substituted DNA was isolated form B. Subtilis and phage PBSH. The three DNA fractions of Different densities (TT, TB, and BB) were irradiated with u.v. (313nm). The number of single‐strand and double‐strand breaks was determined. The breakage rates are given. It was found that in hybrid DNA (TB) double‐strand breaks occur depending linearly on dose. In BB–DNA the observed double‐Strand bresks can be divided into two fractions with a linear and quadratic dose dependence respectively. The results can be explained by assuming intramolecular energy transfer from the BU‐containing strand to its complementary strand.