Simultaneous separation and sensitive determination of free fatty acids in blood plasma by high-performance liquid chromatography
β Scribed by Hironori Tsuchiya; Tokishi Hayashi; Masaru Sato; Mikio Tatsumi; Nobuhiko Takagi
- Publisher
- Elsevier Science
- Year
- 1984
- Weight
- 608 KB
- Volume
- 309
- Category
- Article
- ISSN
- 0378-4347
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Determination of free fatty acids (FFAs) in control serum and human plasma was carried out by highperformance liquid chromatography with electrochemical detection. Peak height for palmitic, palmitoleic, stearic, oleic, linoleic, and arachidonic acids at a detection potential of Ψ415 mV vs a saturate
## Abstract A highly sensitive and simple reversed phase high performance liquid chromatographic (HPLC) method for the quantitative determination of free fatty acids in human serum is presented. The method is based on the direct derivatization of serum fatty acids with 6,7βdimethoxyβ1βmethylβ2(1__H