Simultaneous separate detection of low angle and large angle light scattering in an arc lamp-based flow cytometer

✍ Scribed by Dr. Harald B. Steen

• Publisher: John Wiley and Sons
• Year: 1986
• Tongue: English
• Weight: 487 KB
• Volume: 7
• Category: Article
• ISSN: 0196-4763
• DOI: 10.1002/cyto.990070509

✦ Synopsis

A device is described for simultaneous separate detection of the light scattering of cells at low and large scattering angles in an arc lamp-based flow cytometer with epi-illumination through an oil immersion microscope objective. Light scattering was measured in a dark field configuration that allows separate detection of light scattering greater than 2" and l5", respectively. Dual parameter light scattering histograms of a blood cell suspension containing various types of leukocytes were closely similar to that obtained with a commercial laser-based instrument with light scattering detection at forward and right angles. The sensitivity of the device was sufficient to measure polystyrene particles with 0.25-pm diameter. A potential application may be differentiation of bacteria.