Simultaneous saccharification and fermentation of pretreated Antigonum leptopus (Linn) leaves to ethanol

✍ Scribed by S Hari Krishna; G V Chowdary; D Srirami Reddy; C Ayyanna

• Publisher: Wiley (John Wiley & Sons)
• Year: 1999
• Tongue: English
• Weight: 100 KB
• Volume: 74
• Category: Article
• ISSN: 0268-2575
• DOI: 10.1002/(sici)1097-4660(199911)74:11<1055::aid-jctb148>3.0.co;2-z

✦ Synopsis

Simultaneous sacchari®cation and fermentation (SSF) of alkaline hydrogen peroxide pretreated Antigonum leptopus (Linn) leaves to ethanol was optimized using cellulase from Trichoderma reesei QM-9414 (Celluclast 1 from Novo) and Saccharomyces cerevisiae NRRL-Y-132 cells. Contrary to the sacchari®cation optima (2.5% w/v substrate concentration, 50 °C, 4.5 pH, 40 FPU cellulase g À1 substrate and 24 h reaction time), the SSF optima was found to be somewhat different (10% w/v substrate, 40 °C, 100 FPU cellulase g À1 substrate and 72 h). Better ethanol yields were obtained with SSF compared with the traditional sacchari®cation and subsequent fermentation (S&F) and when the cellulase was supplemented with b-glucosidase.