Simultaneous radioimmunoassay of secretin and gastrin

✍ Scribed by Hsin-Hsiung Tai; William Y. Chey

• Book ID: 102983856
• Publisher: Elsevier Science
• Year: 1976
• Tongue: English
• Weight: 736 KB
• Volume: 74
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(76)90305-5

✦ Synopsis

Radioimmunoassay techniques for the measurement of gastrointestinal hormones have been modified to allow simultaneous assay of both secretin and gastrin in the same plasma samples. The system employs antibodies with high specificity for these two hormones and both 1251-labeted secretin and 1311-labeled gastrin as markers. Separation of the free from the bound form of the hormones was achieved by plasma-and dextran-coated charcoal. The unique separation method has been found to be independent of the uniformity of plasma samples. The sensitivity and the reliability of the simultaneous assays were comparable to those of the single assays. The fasting levels of human plasma secretin and gastrin obtained by the simultaneous assay were 62.9 f 3.6 and 43.5 2 2.8 pg/ml respectively.

MATERIALS

Anfibodies.

Antibodies to secretin were produced and evaluated as previously described (4). Antibodies to gastrin were generated in a fashion