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Simultaneous quantitation of equine cytokine mRNAs using a multi-probe ribonuclease protection assay

โœ Scribed by W.-S Lim; J.F Edwards; N.K Boyd; S.L Payne; J.M Ball


Book ID
117336073
Publisher
Elsevier Science
Year
2003
Tongue
English
Weight
292 KB
Volume
91
Category
Article
ISSN
0165-2427

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partially degraded RNA. The assay involves hybridiza-We have developed a nonisotopic RNase protection tion of RNA samples to radiolabeled antisense RNA assay using RNA probes that are dual-labeled with bioprobe in solution, followed by digestion of unhybridized tin and fluorescein for detection. Thi