Simultaneous quantification of three iridoids in rat plasma after oral administration of Zhi-zi-chi decoction using LC-MS

✍ Scribed by Zhi-Min Long; Kai-Shun Bi; Yan-Shuang Huo; Xiao-Yu Yan; Xu Zhao; Xiao-Hui Chen

• Publisher: John Wiley and Sons
• Year: 2011
• Tongue: English
• Weight: 205 KB
• Volume: 34
• Category: Article
• ISSN: 1615-9306
• DOI: 10.1002/jssc.201100387

✦ Synopsis

Abstract

A sensitive and efficient liquid chromatography–mass spectrometry (LC‐MS) method was developed and validated for the simultaneous determination of geniposide, 6α‐hydroxygeniposide, and genipin gentiobioside in rat plasma. After the addition of internal standard (I.S.) salidroside and acidification (formic acid, 0.1%), plasma samples were carried out by protein precipitation with acetonitrile and separated on a Kromasil C~18~ column (200 mm×4.6 mm, 5 μm) within a runtime of 15.0 min. The linear ranges were 2–250 ng/mL for both 6α‐hydroxygeniposide and genipin gentiobioside and 2–2000 ng/mL for geniposide, respectively. The lower limit of quantification (LLOQ) was 2 ng/mL for all the analytes. The validated method was successfully applied to the pharmacokinetics study of geniposide, 6α‐hydroxygeniposide, and genipin gentiobioside in rats after oral administration of Zhi‐zi‐chi decoction.