𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Simultaneous quantification of polymer and salt in polyethylene glycol / phosphate aqueous two-phase systems by HPLC

✍ Scribed by M. J. Sebastião; J. M. S. Cabral; M. R. Aires-Barros

Book ID
104648709
Publisher
Springer-Verlag
Year
1995
Tongue
English
Weight
362 KB
Volume
9
Category
Article
ISSN
0951-208X

No coin nor oath required. For personal study only.

✦ Synopsis

A simple method for the determination of phase composition in polyethylene glycol / phosphate aqueous biphasic systems was developed. A single HPLC analysis allows the simultaneous quantification of polymer and salt in the top phase. Phosphate concentration in the bottom phase was also accurately determined but the method was not sensitive enough for PEG quantification in this phase.

📜 SIMILAR VOLUMES

Oxidative renaturation of hen egg-white
Oxidative renaturation of hen egg-white lysozyme in polyethylene glycol–salt aqueous two-phase systems
✍ Jorge Lotwin; Eliana De Bernardez Clark 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 354 KB 👁 2 views

Aqueous two-phase systems have been widely used for the separation and concentration of proteins. In this work we investigated the possibility of using aqueous two-phase system for the renaturation of inclusion body proteins by studying the effect of polyethylene glycol (PEG)-salt systems on the oxi