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Simultaneous quantification of four vitamin D metabolites in human serum using high performance liquid chromatography tandem mass spectrometry for vitamin D profiling

✍ Scribed by Silvia Baecher; Andreas Leinenbach; Jo Anne Wright; Stephan Pongratz; Uwe Kobold; Roland Thiele


Book ID
118431875
Publisher
Elsevier Science
Year
2012
Tongue
English
Weight
555 KB
Volume
45
Category
Article
ISSN
0009-9120

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A validated method is described for the simultaneous analysis of PGE 2 , 11-, 12-, and 5-HETEs from cultured cells using HPLC negative electrospray ionization tandem mass spectrometry (LC/MS/MS). This method permits quantification of selected individual arachidonic acid metabolites from cell extract