Simultaneous production of citric acid and erythritol from crude glycerol byYarrowia lipolyticaWratislavia K1

✍ Scribed by Waldemar Rymowicz; Anita Rywińska; Witold Gładkowski

• Book ID: 111491132
• Publisher: Versita
• Year: 2008
• Tongue: English
• Weight: 233 KB
• Volume: 62
• Category: Article
• ISSN: 0366-6352
• DOI: 10.2478/s11696-008-0018-y

✦ Synopsis

Abstract

This study shows a possible microbial process for utilization of crude glycerol generated by the biodiesel industry for citric acid and erythritol production. Simultaneous production of citric acid and erythritol under nitrogen-limited conditions with glycerol as the carbon source was achieved with an acetate negative mutant of Y. lipolytica Wratislavia K1 in fed-batch cultivations. The effect of the initial glycerol concentration (from 30–180 g dm−3) on the citrate and erythritol production was investigated. As a result of the experiments, maximum citric acid production (110 g dm−3) and a very high amount of erythritol (81 g dm−3) were determined after 168 h of fed-batch cultivation with the initial glycerol concentration of 150 g dm−3 and the total glycerol concentration of 250 g dm−3. In addition, the citric acid to isocitric acid ratio of the products from this strain was 35.5:1.