Simultaneous NMR assignment of backbone and side chain amides in large proteins with IS-TROSY

Hydrogen-Bond Detection, Configuration A

Hydrogen-Bond Detection, Configuration Assignment and Rotamer Correction of Side-Chain Amides in Large Proteins by NMR Spectroscopy through Protium/Deuterium Isotope Effects

✍ Aizhuo Liu; Jifeng Wang; Zhenwei Lu; Lishan Yao; Yue Li; Honggao Yan 📂 Article 📅 2008 🏛 John Wiley and Sons 🌐 English ⚖ 382 KB 👁 1 views

## Abstract The configuration and hydrogen‐bonding network of side‐chain amides in a 35 kDa protein were determined by measuring differential and trans‐hydrogen‐bond H/D isotope effects by using the isotopomer‐selective (IS)‐TROSY technique, which leads to a reliable recognition and correction of e