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Simultaneous Measurements of Solvent Dynamics and Functional Kinetics in a Light-Activated Enzyme

โœ Scribed by Durin, Guillaume; Delaunay, Aude; Darnault, Claudine; Heyes, Derren J.; Royant, Antoine; Vernede, Xavier; Hunter, C. Neil; Weik, Martin; Bourgeois, Dominique

Book ID
119204472
Publisher
Biophysical Society
Year
2009
Tongue
English
Weight
778 KB
Volume
96
Category
Article
ISSN
0006-3495

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โœ Tao Ke; Alexander M. Klibanov ๐Ÿ“‚ Article ๐Ÿ“… 1998 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 63 KB ๐Ÿ‘ 2 views

Catalytic activities of โฃ-chymotrypsin and subtilisin Carlsberg in various hydrous organic solvents were measured as a function of how the enzyme suspension had been prepared. In one method, lyophilized enzyme was directly suspended in the solvent containing 1% water. In another, the enzyme was prec