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Simultaneous measurements of fluorescence polarization and quenching: A specially designed instrument and an application to the micro-brownian motion of polymer chains

✍ Scribed by J.P. Bentz; J.P. Beyl; G. Beinert; G. Weill


Publisher
Elsevier Science
Year
1975
Tongue
English
Weight
611 KB
Volume
11
Category
Article
ISSN
0014-3057

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✦ Synopsis


A~tract--Simultaneous measurements of polarization and quenching of fluorescence are used to study the rotational Brownian motion. A modulation device has been built leading to precise measurements of the sum and difference of the polarized components of fluorescence. A comparison is made between this method and the Perrin method for a fluorescent group, free or bound to a polymer, either on a side-chain or in the backbone. Results are discussed in terms of the shape of the autocorrelation function for the rotational micro-Brownian motion of chain molecules.