Isotope dilution gas chromatographic mass spectrometric assays were developed for the concentration of shortchain fatty acids and of lactate in plasma. The assays involve spiking the samples with 'Hand/or "C-labeled internal standards and direct derivatization with 2,4difluoroaniline, using 1,3-dicy
Simultaneous measurement of plasma concentrations and 13C-enrichment of short-chain fatty acids, lactic acid and ketone bodies by gas chromatography coupled to mass spectrometry
β Scribed by N.M Moreau; S.M Goupry; J.P Antignac; F.J Monteau; B.J Le Bizec; M.M Champ; L.J Martin; H.J Dumon
- Book ID
- 108497416
- Publisher
- Elsevier Science
- Year
- 2003
- Tongue
- English
- Weight
- 271 KB
- Volume
- 784
- Category
- Article
- ISSN
- 1873-376X
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## Abstract A simple procedure for the extraction and derivatization of Ξ±βamino acids and Ξ±βketo acids from human plasma is described. This stable isotope dilution method permits the quantification of isotopic enrichment and concentrations of several Ξ±βketo and Ξ±βamino acids simultaneously. The der