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Simultaneous measurement of neutrophil, lymphocyte, and monocyte glutathione by flow cytometry

✍ Scribed by Robert B. Scott; James M. Collin; Sina Matin; Frances White; Paul S. Swerdlow

Publisher: John Wiley and Sons
Year: 1990
Tongue: English
Weight: 363 KB
Volume: 4
Category: Article
ISSN: 0887-8013
DOI: 10.1002/jcla.1860040503

No coin nor oath required. For personal study only.

✦ Synopsis

A flow cytometric method for quantitation of glutathione (GSH) was applied to simultaneous analysis of the major leukocyte types in peripheral blood. Cellular thiols (predominately GSH) were stained with monochlorobimane (MCIB), and thiol fluorescence was measured with a flow cytometer. The fluorescence of the thiols closely reflected the GSH content, as measured by a specific glutathione reductase assay. Fluorescence of indi- vidual cell types could be measured after delineating those cells by their light-scatter characteristics, utilizing dual-angle light scatter for discrimination. By this means, GSH contents of 12.5 k 2.0 nmol/107 neutrophils, 14.5 ? 2.7 nmol/107 monocytes, and 5.0 f 1 .O nmol/107 lymphocytes were found. The results obtained for neutrophils with the flow cytometer were virtually identical with those obtained with chemical assay in purified samples of neutrophils, indicating the validity of the flow cytometric method.

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