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Simultaneous Measurement of Cytochrome P4501A Catalytic Activity and Total Protein Concentration with a Fluorescence Plate Reader

โœ Scribed by S.W. Kennedy; S.P. Jones

Book ID
102559980
Publisher
Elsevier Science
Year
1994
Tongue
English
Weight
467 KB
Volume
222
Category
Article
ISSN
0003-2697

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โœฆ Synopsis

A method for simultaneous measurement of the cytochrome P4501A-associated enzyme, ethoxyresorufin0 -deethylase (EROD), and total protein concentration in liver microsomes is described. EROD assays were carried out in multiwell plates, and the fluorescent product (resorufin) and total proteins were quantified within the same wells with a fluorescence plate reader. Fluorescamine was used for protein assays. ce 1994 Academic Press, Inc.

๐Ÿ“œ SIMILAR VOLUMES

Efficient Analysis of Cytochrome P4501A
Efficient Analysis of Cytochrome P4501A Catalytic Activity, Porphyrins, and Total Proteins in Chicken Embryo Hepatocyte Cultures with a Fluorescence Plate Reader
โœ S.W. Kennedy; S.P. Jones; L.J. Bastien ๐Ÿ“‚ Article ๐Ÿ“… 1995 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 576 KB

An efficient method for measuring polychlorinated biphenyl-mediated induction of cytochrome P4501A, using the ethoxyresorufin-O-deethylase (EROD) assay, and total porphyrins in chicken embryo hepatocyte cultures is described. Hepatocytes were cultured in \(48-\) well plates, assays were carried out