Simultaneous Kinetic-Spectrophotometric Determination of Bilirubin and Albumin in Human Serum Using the Diazo Method

✍ Scribed by A. Sevillanocabeza; M.C. Alberolagarbi; P. Campinsfalco

• Publisher: Elsevier Science
• Year: 1994
• Tongue: English
• Weight: 677 KB
• Volume: 50
• Category: Article
• ISSN: 0026-265X
• DOI: 10.1006/mchj.1994.1053

✦ Synopsis

A kinetic-spectrophotometric study based on the reaction of bilirubin with (p)-diazobenzene sulfonic and sulfanilic acid with sodium nitrite is described. The simultaneous addition of caffeine reagent, diazo reagent, and alkaline tartrate, reagents used in the determination of bilirubin, in the presence of albumin leads to a time evolution of absorption spectra. This evolution is proportional to albumin concentration and constitutes the basis of a new method (based on the diazo method) for the determination of albumin. Albumin concentrations determined from the proposed method are compared with those obtained by using the bromcresol green method. The method is also applied to the simultaneous determination of bilirubin and albumin in human serum samples. A study of the accuracy in the determination is carried out by means of Youden's method and the standard additions method. It is conclusively shown that the method is not affected by constant or proportional bias errors. Finally, the interference caused by hystidine, tyrosine, lysine, ascorbic acid, and hemoglobin is also studied. 1994 Academic Press. Inc.