Simultaneous isotopic and nonisotopic in situ hybridization histochemistry with cRNA probes
β Scribed by Julie A. Kerner; David G. Standaert; John B. Penney Jr.; Anne B. Young; G.Bernhard Landwehrmeyer
- Book ID
- 117769067
- Publisher
- Elsevier Science
- Year
- 1998
- Tongue
- English
- Weight
- 683 KB
- Volume
- 3
- Category
- Article
- ISSN
- 1385-299X
No coin nor oath required. For personal study only.
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In situ hybridization histochemistry is a valuable technique for localizing specific messenger RNA (mRNA) and detecting changes in gene expression. Generally, the mRNA of interest has been detected by probes obtained from cloned DNA and labelled to high specific activity by nick translation. Such pr