Simultaneous intracellular delivery of targeting antibodies and functional nanoparticles with engineered protein G system
✍ Scribed by Yong Taik Lim; Mi Young Cho; Jung Min Lee; Sang Jeon Chung; Bong Hyun Chung
- Book ID
- 104003762
- Publisher
- Elsevier Science
- Year
- 2009
- Tongue
- English
- Weight
- 671 KB
- Volume
- 30
- Category
- Article
- ISSN
- 0142-9612
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✦ Synopsis
Cellular internalization of functional nanoparticles that have optical and magnetic properties is very important in the cellular imaging and manipulation of specifically targeted biomolecules. In this study, a robust method to deliver functional nanoparticles and targeting antibodies into cells was suggested. The engineered protein G system, which contains an affinity tag and a cell penetration peptide in the N- and C-terminals, respectively, can capture surface-modified nanoparticles and antibodies without chemical reaction, and then non-invasively deliver them into the cells. Finally, gold-coated iron oxide nanoparticle/engineered protein G hybrid systems were successfully employed as multifunctional cargo systems for the targeting, imaging, and manipulation of mitochondria.