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Simultaneous intracellular and extracelular pH measurement in the heart by 19F NMR of 6-fluoropyridoxol

✍ Scribed by Sandeep Hunjan; Ralph P. Mason; Vimal D. Mehta; Padmakar V. Kulkarni; Subramanian Aravind; Veeva Arora; Peter P. Antich

Publisher: John Wiley and Sons
Year: 1998
Tongue: English
Weight: 617 KB
Volume: 39
Category: Article
ISSN: 0740-3194
DOI: 10.1002/mrm.1910390407

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✦ Synopsis

6-Fluoropyridoxol (6-FPOL) was evaluated as a simultaneous indicator of intracellular and extracellular pH and, hence, pH gradient in perfused rat hearts. After infusion, 19F NMR spectra rapidly showed two well-resolved peaks assigned to the intracellular and extracellular compartments, and pH was calculated on the basis of chemical shift with respect to a sodium trifluoroacetate standard. To demonstrate use of this molecule, dynamic changes in myocardial pH were assessed with a time resolution of 2 min during respiratory and metabolic alkalosis or acidosis and ischemia. For a typical heart, intracellular pH (pHi) = 7.14+/-0.01 and extracellular pH (pHe) = 7.52+/-0.02. In response to metabolic alkalosis, pHi remained relatively constant and the pH gradient increased. In contrast, respiratory challenge caused a significant increase in pHi. Independent measurements using pH electrodes and 31P NMR confirmed validity of the 19F NMR results.

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