Simultaneous preparation of nuclear DNA,
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Michael C. MacLeod; Betty K. Mansfield; Anne Huff; James K. Selkirk
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Article
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1979
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Elsevier Science
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English
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Nuclei from hamster embryo fibroblasts treated with radioactive benzo(a)pyrene were lysed in 6 M guanidine, and nuclear macromolecules were separated by isopycnic centrifugation in CslSOI. Control experiments showed that cross-contamination of the RNA, DNA, and protein fractions was less than 2% of