Simultaneous efflux of endogenous D-ser and L-glu from single acute hippocampus slices during oxygen glucose deprivation
✍ Scribed by Daniel L. Kirschner; Ann L. Wilson; Kelly L. Drew; Thomas K. Green
- Book ID
- 102382660
- Publisher
- John Wiley and Sons
- Year
- 2009
- Tongue
- English
- Weight
- 251 KB
- Volume
- 87
- Category
- Article
- ISSN
- 0360-4012
No coin nor oath required. For personal study only.
✦ Synopsis
Abstract
D‐serine and L‐glutamate play crucial roles in excitotoxicity through N‐methyl‐D‐aspartate receptor coactivation, but little is known about the temporal profile of efflux during cerebral ischemia. We utilized a newly designed brain slice microperfusion device coupled offline to capillary electrophoresis laser‐induced fluorescence to monitor dynamic efflux of endogenous D‐ser and L‐glu in response to oxygen glucose deprivation (OGD) in single acute hippocampus slices. Efflux profiles with 2‐min temporal resolution in response to 24‐min OGD show that efflux of D‐ser slightly precedes efflux of L‐glu by one 2‐min sampling interval. Thus both coagonists are available to activate NMDA receptors by the time when glu is released. The magnitude of D‐ser efflux relative to baseline values is, however, less than that for L‐glu. Peak efflux during OGD, expressed as pre‐OGD baseline values, was as follows: D‐ser 254% ± 24%, L‐glu 1,675% ± 259%, L‐asp 519% ± 128%, and L‐thr 313% ± 33%. L‐glutamine efflux was shown to decrease significantly in response to OGD. The microperfusion/CE‐LIF approach shows several promising attributes for studying endogenous chemical efflux from single, acute brain slices. © 2009 Wiley‐Liss, Inc.