Simultaneous determination of vitexin-2′′-O-glucoside, vitexin-2′′-O-rhamnoside, rutin, and hyperoside in the extract of hawthorn (Crataegus pinnatifida Bge.) leaves by RP-HPLC with ultraviolet photodiode array detection
✍ Scribed by Shan Cheng; Feng Qiu; Jia Huang; Junqi He
- Book ID
- 102440695
- Publisher
- John Wiley and Sons
- Year
- 2007
- Tongue
- English
- Weight
- 386 KB
- Volume
- 30
- Category
- Article
- ISSN
- 1615-9306
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✦ Synopsis
Abstract
RP‐HPLC with UV photodiode array detection (UV‐DAD) was developed and validated for the simultaneous determination of vitexin‐2′′‐O‐glucoside, vitexin‐2′′‐O‐rhamnoside, rutin, and hyperoside in the extract of hawthorn (Crataegus pinnatifida Bge.) leaves. The analytes of interest were separated on a Diamonsil C~18~ column (250×4.6 mm id, 5 μm) with the mobile phase consisting of THF/ACN/methanol/0.05% phosphoric acid solution (pH 5.0) (18:1:1:80 v/v/v/v). The flow rate was set at 1.0 mL/min and the eluent was detected at 340 nm for the four flavonoids. The method was linear over the studied range of 1.00–100 μg/mL for the four analytes of interest with the correlation coefficient for each analyte greater than 0.999. The LOD and LOQ were 0.03 and 0.10 μg/mL, 0.03 and 0.10 μg/mL, 0.05 and 0.15 μg/mL, 0.10 and 0.30 μg/mL for vitexin‐2′′‐O‐glucoside, vitexin‐2′′‐O‐rhamnoside, rutin, and hyperoside, respectively. The optimized method was successfully applied to the analysis of four important flavonoids in the extract of hawthorn leaves. The total amounts of the four flavonoids were 22.2, 62.3, 4.27, and 8.24 mg/g dry weight for vitexin‐2′′‐O‐glucoside, vitexin‐2′′‐O‐rhamnoside, rutin, and hyperoside in the extract of hawthorn leaves, respectively.