Simultaneous determination of thyroxine and triiodothyronine in pharmaceutical formulations using capillary electrophoresis with amperometric detection

✍ Scribed by Yan Sun; Xiaoyin Zhao; Ping Li; Guoyue Shi; Tianshu Zhou

• Publisher: John Wiley and Sons
• Year: 2010
• Tongue: English
• Weight: 162 KB
• Volume: 33
• Category: Article
• ISSN: 1615-9306
• DOI: 10.1002/jssc.201000077

✦ Synopsis

Abstract

A simple, reliable and repeatable method, based on CE with amperometric detection has been developed for the simultaneous separation and determination of thyroxine (T~4~) and 3,3′,5‐triiodothyronine (T~3~). A carbon disk electrode was used as working electrode in this system and exhibited a good response at 1.05 V (versus Hg/Hg~2~Cl~2~, 3 mol/L KCl) for two analytes. The CE separations were investigated in detail. At 21 kV of applied voltage, the two compounds were separated within 11 min in 0.05 mol/L Na~2~B~4~O~7~‐NaOH buffer (pH 11.3). And the detection limits were as low as 1.0×10^−7^ mol/L for T~4~ and 8.5×10^−8^ mol/L for T~3~ (S/N=3), respectively. The proposed method was applied to analyze T~4~ and T~3~ in pharmaceutical formulations, and the results were satisfactory.