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Simultaneous determination of the three major monoamine metabolites in brain tissue and body fluids by a mass fragmentographic method

✍ Scribed by Carl-Gunnar Swahn; Bengt Sandgärde; Frits-Axel Wiesel; Göran Sedvall

Publisher
Springer
Year
1976
Tongue
English
Weight
464 KB
Volume
48
Category
Article
ISSN
0033-3158

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✦ Synopsis

A mass fragmentographic method for the simultaneous determination of 4-hydroxy-3-methoxyphenylacetic acid (HVA), 4-hydroxy-3-methoxyphenylethylene glycol (MOPEG), and 5-hydroxyindole-3-acetic acid (5-HIAA) was described. Deuterated analogues of the compounds were used as internal standards. The specificity was proved by multiple ion analysis. The experimental error was below 7 % when applied to the analysis of human lumbar cerebrospinal fluid, urine, or rat brain tissue. In cerebrospinal fluid the major part of the monoamine metabolites occurred in the free form. In rat brain and human urine considerable amounts of conjugated HVA was found.

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